Why is the optimum pH of amylase 7?
Why is the optimum pH of amylase 7?
pH 7 is the optimum pH for amylase. Above pH 7, the activity of amylase rapidly decreases beacuse the concentration of H+ ions (or protons) is too low. This change in [H+] alters the interactions between the variable R-groups of the amino acids in the protein chains that make up the enzyme.
What is the optimal pH and temperature for amylase?
Amylase catalyzes the hydrolysis (breaking down) of starch, glycogen and related polysaccharides into more simple and readily usable forms of sugar. AMYLASE has an OPTIMAL RANGE of pH and Temperature which is pH = 7 (neutral) and 37 degrees C.
What is the best temperature for amylase activity?
37 °C
What would happen if more enzyme was added?
By increasing the enzyme concentration, the maximum reaction rate greatly increases. Conclusions: The rate of a chemical reaction increases as the substrate concentration increases. Enzymes can greatly speed up the rate of a reaction. However, enzymes become saturated when the substrate concentration is high.
What is the problem in determining rates at low substrate concentration?
For low substrate concentrations (relative to the Km), depletion of the substrate causes the reaction to slow down more than at higher substrate concentration, so a low enzyme concentration is needed to maintain the initial rate long enough for the initial rate measurement to be made.
Why are low concentrations of enzymes effective?
In most situations, decreasing enzyme concentration has a direct influence on enzyme activity because each enzyme molecule is able to catalyze only one reaction at a time. In general, one enzyme binds to one substrate in order to lower the activation energy for one chemical reaction.
What happens to enzymes at low temperature?
At low temperatures enzymes are simply inactive. This increases the frequency of collisions and the formation of enzyme-substrate complexes. Therefore as the temperature is increased the enzyme activity and the rate of reaction increases.