Why is the acid fast stain a useful diagnostic procedure?
Why is the acid fast stain a useful diagnostic procedure?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses….
What is the purpose of steaming in AFB staining?
The heat will prevent the cells from being destained using acid-alcohol. Because these cells hold fast to the primary stain with acid alcohol treatment, they are termed acid fast positive. All other cells will easily be destained by the addition of acid alcohol and are termed non-acid fast.
Why is time important in simple staining?
Why is time an important factor in simple staining? Time is important because it creates a contrast between the bacteria and the stain. If you over or under stain you won’t be able to see bacteria. A properly prepared bacterial smear would mean the bacteria are evenly spread out and properly fixed.
Which of the four staining procedures is the most important?
The Gram stain is the most important staining procedure in microbiology.
Why do we fix the slide before staining?
The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure.
What is the purpose of using Leishman’s stain?
Leishman stain, also known as Leishman’s stain, is used in microscopy for staining blood smears. It is generally used to differentiate between and identify white blood cells, malaria parasites, and trypanosomas.
What should you do if your slide looks drastically different from your slide?
What should you do if your slide looks dramatically different from the slide in your lab manual? You probably should move the slide around a bit or change to a different power objective to find the correct tissue or cell type on the slide.
Why do you start at the lowest power lens when viewing slides?
Why do you need to start with 4x in magnification on a microscope? The 4x objective lens has the lowest power and, therefore the highest field of view. As a result, it is easier to locate the specimen on the slide than if you start with a higher power objective.