When preparing a wet mount specimen for viewing?
When preparing a wet mount specimen for viewing?
In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Place a sample on the slide. Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent.
What is the order of the threads from top to bottom?
As the observer focuses down, first the top thread comes into focus, then the middle one, and finally the bottom one.
When working with a microscope what term is used to indicate that if an object is in focus at low power magnification it will also be in focus at high power?
Cards In This Set
Front | Back |
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What word is used to indicate that if the object is in focus at low power, it will also be in focus at high power? | Parfocal |
What adjustment knob is used with high power? | Fine adjustment knob |
If a Euglena is swimming to the left, which way should you move your slide to keep it in view? | Right |
Does a wet mount require dyes 12 hours before viewing?
A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen. oil immersion increases the resolving power of the specimen.
Why do you need to make a wet mount of a live sample right after you get it?
In a wet mount, the specimen is suspended in a drop of liquid (usually water) located between slide and cover glass. The water refractive index of the water improves the image quality and also supports the specimen.
What lens can get dirty with oil if you move the revolving nosepiece in the wrong direction?
What lens could get dirty with oil if you move the revolving nosepiece in the wrong direction after viewing under oil immersion? add a drop of immersion oil before rotating the 100x lens into position.
What is the proper procedure for preparing a wet mount?
Wet Mount:
- Place a drop of fluid in the center of the slide.
- Position sample on liquid, using tweezers.
- At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop.
- Lower the cover slowly, avoiding air bubbles.
- Remove excess water with the paper towel.