Why should you use an inoculating needle when making smears from solid media an inoculating loop from liquid media?
Why should you use an inoculating needle when making smears from solid media an inoculating loop from liquid media?
An inoculating loop from liquid media? Solid media is more dense over a smaller area so an inoculating needle is used to retrieve the specimen so not too much is transferred, while a liquid medium features a more spread out specimen so the inoculating loop can collect more liquid and enough specimen to observe.
How do you Sterilise an inoculating loop?
The inoculating loop is sterilised by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat. In this way all contaminants on the wire are incinerated. Never lay the loop down once it is sterilised, or it may again become contaminated.
Why can’t the inoculating loop be used for stab inoculation?
It is important to use a needle rather than an inoculating loop because the needleis used to transfer the specimen to the soft agar medium. The inoculating loop isused to transfer specimens in a liquid medium or plating. Using the needle willcreate more growth to occur along the stab line.
Why is inoculating loop flamed?
The most important tool for transferring cultures is the wire inoculating needle or loop. It can be quickly sterilized by heating it to red hot in a Bunsen burner flame. Always flame the loop immediately before and after use! Allow it to cool before picking up an inoculum of bacteria (or you will kill the bacteria).
How much time is required to properly sterilize inoculating loops and needles?
20-30 seconds
When flaming your loop What direction should you always flame?
Flame your loop (or needle) from the end of the handle (the end nearest the tip, not the end you’re holding) to the tip of the loop. Be sure that each segment of metal glows orange before you move the next segment into the flame. Be careful; the metal will get hot. 7.
What is the flaming loop?
The inoculating loop is usually used for making transfers of bacterial cultures. Instructions for the proper technique used to flame a loop with a Bunsen burner are provided on the following page.
What would happen if you were to insert a red hot loop for picking the bacterial culture?
What would happen if you If you were to insert a red hot loop for picking the bacterial culture? The hot loop may create aerosols when it touches the culture. The airborne bacteria would be harmful to yourself and others.
How would you cool an inoculation loop without contaminating it?
Allow the loop to cool a few seconds to avoid killing the inoculum. Partially lift the lid of the plate culture and open it just enough to insert the inoculation loop. Do not completely open the lid and expose the surface to the air. Touch the loop to an area of the agar with no growth in order to cool down the loop.
Why must the loop be flamed before entering a culture to be isolated?
Why is the loop flamed before it is placed in a culture tube? The loop is flamed before entering a culture tube to ensure that no contaminating microbes are introduced in to the culture. The loop is flamed afterward so that no culture microorganisms are introduced into the working environment.
How is the loop flamed during aseptic transfers?
Significance of Flaming: When transferring a culture from a plate, cool the loop by touching on the very edge of agar. When transferring from a broth, the red-hot loop will make a sizzling noise as soon as you insert it into the culture.
What is the purpose of flame sterilizing the inoculating loop in between quadrants?
Flame the loop or use a new disposable loop after you streak each quadrant. Using a new or sterilized loop allows you to effectively dilute the inoculum on the plate and obtain isolated colonies by spreading the inoculum thinner and more evenly.
Why is it best to incinerate a loop from the handle toward the loop tip rather than flaming the loop part first?
Why is it best to incinerate a loop from the handle toward the loop tip, rather than flaming the loop part first? To reduce the aerosolization of microbes from the loop into the air. What are the most commonly used instruments for transferring microbes between all media types?
What are the steps of aseptic technique?
Aseptic preparation may involve:
- disinfecting a patient’s skin using antiseptic wipes.
- sterilizing equipment and instruments before a procedure.
- keeping sterilized instruments inside plastic wrappers to prevent contamination before use.