How accurate is a pipette?

How accurate is a pipette?

A pipette is accurate to the degree that the volume delivered is equal to the specified volume. … A pipette can be consistently inaccurate but this inaccuracy could be very precise, for example if a pipette reads consistently low.

What is the Z factor in pipette calibration?

The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity. These factors are usually combined to give the Z factor, used in calculation of volume of water.

Why is accurate pipetting important?

Lab Report 2: Impact of Pipetting Technique. The pipette is a reliable precision instrument that has been used and trusted for many years. … With increasing demand for accuracy, quality and productivity the importance of understanding and developing optimal pipetting technique becomes imperative.

What is pipetting error?

Sources of Error in Pipetting. Failure of properly align the meniscus with the volume mark. … Allowing the tip of the pipet to rise above the liquid in the container usually causes the liquid to be sucked into the pipet bulb. A broken or chipped pipet can reduce the amount of liquid held after transfer.