What is high performance liquid chromatography used for?

What is high performance liquid chromatography used for?

High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.

What is the principle of high performance liquid chromatography?

That makes it much faster. All chromatographic separations, including HPLC operate under the same basic principle; separation of a sample into its constituent parts because of the difference in the relative affinities of different molecules for the mobile phase and the stationary phase used in the separation.

What is the stationary phase in high performance liquid chromatography?

Stationary Phases for Gas–Liquid Chromatography In liquid–liquid chromatography the stationary phase is a liquid film coated on a packing material, typically 3–10 μm porous silica particles. Because the stationary phase may be partially soluble in the mobile phase, it may elute, or bleed from the column over time.

Why HPLC is called High Performance?

HPLC is unique highly sophisticated chromatographic equipment used to separate compounds that are dissolved in a solution. This is the reason why it is called High performance LIQUID chromatography. Its mobile phase moves due to gravity.