What are the 4 types of chromatography?

What are the 4 types of chromatography?

There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography.

Is HPLC quantitative or qualitative?

Quantitative and Qualitative analysis of HPLC and GC. Two situations exist for qualitative analysis in HPLC & GC:  The sample components are known and peaks need to be assigned. By injecting standards of the pure compound assign the peaks in the chromatogram based on the retention time of the standard.

What is the basic principle of HPLC?

HPLC utilizes different types of stationary phase (typically, hydrophobic saturated carbon chains), a pump that moves the mobile phase(s) and analyte through the column, and a detector that provides a characteristic retention time for the analyte.

How do you handle HPLC instruments?

However, smaller particles have greater resistance to flow, so higher pressures are needed to create the desired solvent flow rate. Pumps and columns designed to withstand high pressure are necessary. This was called high pressure liquid chromatography, or HPLC.

Why is HPLC used for drug analysis?

The purpose high performance liquid chromatography (HPLC) analysis of any drugs is to confirm the identity of a drug and provide quantitative results and also to monitor the progress of the therapy of a disease.

How does separation occur in HPLC?

General Scheme. Components within a mixture are separated in a column based on each component's affinity for the mobile phase. So, if the components are of different polarities and a mobile phase of a distinct polarity is passed through the column, one component will migrate through the column faster than the other.

How many types of HPLC are there?

There are two elution types: isocratic and gradient. In the first type constant eluent composition is pumped through the column during the whole analysis. In the second type, eluent composition (and strength) is steadily changed during the run.

What is peak area in HPLC?

The area under a peak [peak area count] is a measure of the concentration of the compound it represents. This area value is integrated and calculated automatically by the computer data station. In this example, the peak for acrylamide in Sample A has 10 times the area of that for Sample B.

What is difference between HPLC and GC?

HPLC vs GC HPLC and GC are both methods of separation of compounds from a mixture. Whereas HPLC applies to constituents that are fluids, GC is used when the compounds are gaseous or can be vaporized during separation process. While HPLC refers to High Pressure Liquid Chromatography, GC is simply Gas Chromatography.

What is the working principle of HPLC?

HPLC works on the principle that some molecules take longer than others to pass through a chromatography column. This depends on the affinity of the molecule with the mobile phase (liquid or gas) and the stationary phase (solid or liquid).