How do you make tissue homogenate?

How do you make tissue homogenate?

Preparation of CNS tissue homogenate: Prepare a 10–20% brain tissue homogenate – 0.6 g tissue and 2.4 mL of diluent. Leave the selected antibiotic to be in contact with the homogenate for 1 hour. Centrifuge the tissue homogenate under refrigeration, for 30 minutes at 3,000 rpm (1,400 g).

What is tissue homogenate?

homogenate (plural homogenates) Any material obtained by homogenization. (biology) The slurry of tissues and cells which results when cell structure has been mechanically disrupted.

What is liver homogenate?

For example, a liver homogenate is a liver tissue in which its cells have been mechanically disrupted, releasing the organelles and cytoplasm.